Given the TLC plate above, and the one with urine samples below, determine which

Question

Given the TLC plate above, and the one with urine samples below, determine which patients, if any, have an excess of cysteine (indicative of Cystinuria) and which, if any, have an excess of phenylalanine (indicative of PKU, Phenylketonuria). On a separate sheet of paper (either typed or written clearly), write a persuasive Results and Discussion section presenting the results and identifying the amino acids present in the patients' urine. Provide your data/evidence and logic clearly and thoroughly so that another scientist would be convinced of your conclusion. The presence of certain amino acids in urine can be indicative of medical problems. To determine whether a patient's urine contained these amino acids, a laboratory scientist ran two TLC plates, one with standards, and another with urine samples from three patients. The plates were developed with a reagent that makes a colored compound in the presence of amino acids. This laboratory scientist forgot to label her standards. Given this TLC plate, determine which lane was for the cysteine standard and which the phenylalanine standard.

Explanation / Answer

5. The spot on the left side which has low Rf value is due to the highly polar Cystine which will interact more with the silica and will be reluctant to move up alongwith the solvent on the silica plate and the spot on the right with higher Rf is due to less polar phenylalanine.

Please Note:

if you would like the answer for b) and c) too, upload the question the TLC which is mentioned to have been there above b. Infact, show the complete question including 4a.

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