Justice tics vison Questions 1-26 are worth 2 points each. dissoned but only par
ID: 1065962 • Letter: J
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Justice tics vison Questions 1-26 are worth 2 points each. dissoned but only partiary ioniaed a-amino group So positive charge enryme-linked immunosorbent atsay negative charge anion exchange 1. antibodies to the protein being assayed are avalable, axn) can be developed. 2. In order for DEAE to act as an anion exchanger, it must have a 3. In chromatography, a protein mixture must be applied to the column at a low pH so that the proteins will have a net positive charge and bind to the column. 4. In sos-PAGE electrophoresis, disulfide anked protein subunits are separated by first reacting them wnh 5. The 104.5 bond angle in the water molecule is the result of the of electron orbitals around oxygen. 6. The polarity of the o bond is caused by the x of oygen. 7. A strong acid is completely ionized in water, whereas a weak add is & Asolution of buffer at a pH of 8.5 with a pK of 7.5 would have more capacity to buffer the addition of 9. When a peptide bond is formed, an reacts with a carboxylate group. 10. In the peptide Trp-Ser-Val, valine is at the 11. At a pH above its pk, the phenolic group of tyrosine is 12. At a pH below its pk, the a-amino group of lysine is 13. The net charge of the zwitterion form of Gly is 14. In the tripeptide Trp-Val Phe, the N-terminal residue is 15. In the tripeptide Lys-Pro-Ile, there are charged groups at pH 7.Explanation / Answer
enzyme-linked immunosorbent assay---------------ANSWER
positive charge---------------ANSWER
chromatography is a method of fractionating a protein mixture according to the different polarities of the protein
tetrahedral arrangement-answer---------------ANSWER
Higher electronegativity--------------answer
A strong acid is completely ionized in water, whereas a weak acid is ______.
only partially ionized-------------answer
-answer: acid
answer: C-terminus
ELISA is a famous organization of "wet-lab" sort expository natural chemistry measure that uses a strong stage catalyst immunoassay (EIA) to distinguish the nearness of a substance, more often than not an antigen, in a fluid example or wet specimen.
The ELISA has been utilized as a symptomatic apparatus as a part of pharmaceutical and plant pathology, and additionally a quality-control check in different enterprises.
Antigens from the example are connected to a surface. At that point, a further particular immunizer is connected over the surface so it can tie to the antigen. This immune response is connected to a compound, and, in the last stride, a substance containing the catalyst's substrate is included. The ensuing response creates a perceptible flag, most normally a shading change in the substrate
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Chromatography is a flexible strategy for isolating a wide range of sorts of concoction blends. In this lesson, take in the diverse sorts and employments of the system.
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2-Mercaptoethanol is the synthetic compound with the recipe HOCHCHSH. ME or ME, as it is usually contracted, is utilized to decrease disulfide bonds and can go about as a natural cancer prevention agent by searching hydroxyl radicals
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In any case, on the grounds that the two non-holding sets stay nearer to the oxygen iota, these apply a more grounded shock against the two covalent holding sets, adequately pushing the two hydrogen iotas nearer together. The outcome is a contorted tetrahedral plan in which the H—O—H edge is 104.5°
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peptide bond is a compound bond framed between two atoms when the carboxyl gathering of one particle responds with the amino gathering of the other particle, discharging an atom of water (H2O). This is a lack of hydration combination response (otherwise called a buildup response), and more often than not happens between amino acids .
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