I made a fluorescein solution in dilute base. First you should look up the absor

Question

I made a fluorescein solution in dilute base. First you should look up the absorbance and emission wavelengths for fluorescein. Give the wavelengths found and a reference for your source. Initially, this solution will be highly fluorescent. Under the following changing conditions, what will happen to the fluorescent signal: increase or decrease or have no change? For each part, give a one sentence explanation for your response. A tungsten lamp was used in place of the xenon lamp. A glass cuvette was used in place of the quartz. The solution is degassed so that no gases are dissolved in the solution.

Explanation / Answer

1) a) The peak absorption wavelengths is 480 nm
The peak emission wavelength is 525 nm

2)
b)The signal will absorb energy and will probably dispaly yellow solution when tungsten lamp is used in place of Xenon. So, there will be an increase
c) Signal will transmit its energy when glass cuvette is used in place of quartz. So, it will decrease
d) There will be no prominent influence on signal if the solution is degassed

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