Is the effect of the N155T mutation what you would expect for a residue that mak

Question

Is the effect of the N155T mutation what you would expect for a residue that makes up part of the oxyanion hole? How do the reported values of K_cat and K_M support your answer? Check all that apply. The oxyanion is formed after S goes away. K_cat should be reduced due to the gain of enthalpic stabilization of the transition state. For a mutation of a residue that only interacts with the oxyanion intermediate, one would expect K_M to change significantly. The oxyanion is formed after S binds. K_cat should be reduced due to the loss of enthalpic stabilization of the transition state. For a mutation of a residue that only interacts with the oxyanion intermediate, one would not expect K_M to change significantly. Assuming that the T155 side chain cannot H-bond to the oxyanion intermediate, by how much (in kJ/mol) does N155 appear to stabilize the transition state at 37 degree C? Express your answer to two significant figures and include the appropriate units.

Explanation / Answer

s the effect of the N155T mutation what you would expect for a residue that makes up the oxyanion hole?

The oxyanion hole is formed after S (Serine binds.

For a mutation of a residue that only interacts with the oxyanion intermediate.

You would not expect Km to change significantly.

Kcat should be reduced due to the loss of enthalpic stability of the transition state.

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By how much does N155 stabilize the transition state at 37ºC?

rate enhancement

= Kcat N155 / Kcat T155

dGº = 20 kJ/mol --------------ANSWER

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