The suggested procedure for spotting a sample on a TLC plate involves transferri

Question

The suggested procedure for spotting a sample on a TLC plate involves transferring a droplet of concentrated solution from a capillary onto the plate, allowing it to dry, then repeating the process over the existing spot. Why is it advised to spot multiple times? To achieve a high concentration of sample in a large spot. To facilitate visualization by having a higher compound concentration. To prevent the solvent in the developing chamber from evaporating. To fill the capillary almost instantly by capillary action

Explanation / Answer

we should repet it multiple times for visualisation process.

Answer: to facilitate visualisation by having a higher compound concentration

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