4. Upon completion of a laboratory experiment, a student has removed their lab c

Question

4. Upon completion of a laboratory experiment, a student has removed their lab coat, safety glasses, and gloves. The student realizes they forgot to quench and dispose of an aqueous HCl solution, so they add a solution of aqueous NaOH into the HCl solution, and pour the contents into the organic waste. Please list all the safety issues (if any) in this scenario. (10 points) 5. Based on the melting point ranges in the scheme above, what can be implied about the purity of sample A? (10 points) 6. List two common uses for Thin Layer Chromatography (TLC) 2 Points 6b) What are two common adsorbents used in column chromatography? How are they different? 2 Points 6c). Give two methods to visualize spots after they were developed on a TLC plate 2 Points 6d. Define Rfvalue and calculate Rf values for the reactant (A) and product (B) spots on the TLC plate shown. 4 points

Explanation / Answer

6 ) common uses of TLC we have different uses among that the most important uses of TLC is used to

Purity of any sample : Purity of sample can be carried out with TLC. Direct comparison  is done between the sample and the standard or authentic sample; if any impurity is detected, then it shows extra spots and this can be detected easily.

Identification of compounds: Thin layer chromatography can be employed in purification, isolation and identification of natural products like volatile oil or essential oil, fixed oil, waxes, terpenes, alkaloids, glycosides, steriods.

Examination of reactions: Reaction mixture can be examined by Thin layer chromatography to access whether the reaction is complete or not. This method is also used in checking other separational processes and purification processes like distillation, molecular distillation.

6 b ) generally we use silica gel, aluminium oxide (alumina), or cellulose as a common adsorbents . these two adsorbents are different because silica gel is acting as a acidic adsorbent and aluminium oxide (alumina) is acting as a basic adsorbent .

6 c ) methods to visualise the spots after they were developed on the TLC plate   Visualization of colored compounds is simple – the spots can be directly observed after development. Because most compounds are colorless however, a visualization method is needed so some of the visualization methods include

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