1. (3 points) In one or two paragraphs, describe how you used the results of the

Question

1. (3 points) In one or two paragraphs, describe how you used the results of the three assays to identify solutions A, B, C, and D as glucose, fructose, lactose, and sucrose. Be sure to state which is which. Be sure to correlate the structure of each sugar with the chemical feature that each assay tests for. Be sure to state the identity of the four unknowns (A – D) in your analysis. If one or more of the assays did not work, or there was some other problem with your data, describe the results you were able to obtain, and then explain what you would have expected to see if everything had worked perfectly.

2. (2 points) Based on the results you obtained in the lab, what can you conclude about the carbohydrate composition of honey? In other words, what type of sugars are in honey? In addition to this type of sugar, are there other type(s) of sugars that could also be present in honey? Your answer should be one or two paragraphs.

3. (1 point) In a paragraph, describe how your lab results allowed you to determine which corn syrup brand contains high-fructose corn syrup.

4. (1 point) The enzyme ?-galactosidase can hydrolyze the disaccharide allolactose into glucose and galactose. If you want to monitor the progress of allolactose’s hydrolysis reaction, which assay will work the best? Explain your reasoning.

Explanation / Answer

1 more information is needed for each question

2Honey is composed of 76% sugars , 18% water and the remaining 6% other ingredients. Among sugars fructose is the major component (38-55%). Glucose is up to 31% and the remaining include sucrose ,maltose and other complex carbohydrates.

3. Corn syrup is essentially 100% glucose . HFCS is derived from corn starch. To make HFCS, enzymes are added to corn syrup to convert some of the glucose molecules into another simplest sugar unit - fructose. Thus HFCS is high in fructose compared to corn syrup that contains 100% glucose. Thereby from the composition of corn syrup we can determine whether it contain HFCS or not.

4. The progress of the reaction can be monitored by means of capillary electroforesis.the sample is allowed to react withNa2HPO4 ,MgCl2 and galactosidaze. The sample is

Taken out at at various time

And derivatization. F

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