3. (46 pts) Answer True or False for each of the following questions a) The most

Question

3. (46 pts) Answer True or False for each of the following questions a) The most commonly seen form of DNA double helix in the cell is the A form helix. b) In E coli, the DNA double helix is a circular molecule that is twisted into a superhelix by the process of supercoiling. e) In DNA replication, one of the two chains of each daughter DNA molecule is newly synthesized, and the other is from the parent DNA d) Bases stacked in a double helix absorb more UV light than bases in a single-stranded molecule e) Many DNA polymerases have nuclease activity, allowing the removal of mismatched bases t Translation begins near promoter sites and ends at terminator sites. g) Prokaryotic mRNA undergoes posttranslational processing, but there is no posttranslational processing in eukaryotie mRNA h) The anticodon, consisting of three bases in transfer RNA, recognizes a complementary 3 base sequence in the mRNA called the codon. i) The genetic code is overlapping and non-degenerate. j) The restriction enzymes recognize cleavage sites of 4 to 8 nucleotides in length that are palindromic. k) Fluorescence detection of oligonucleotide fragments produced by the dideoxy method requires radioactive labeling of the nucleotides. l) PCR cycles will amplify the target sequence (short strand) exponentially and the parent sequence (long strand) arithmetically m) If the concentration of salt in solution increases, the melting temperature of DNA decreases. n) As the percentage of the G-C base pairs increases, the melting temperature of DNA decreases. o) In the lung, the presence of higher concentration of O2 promotes the release of H' and CO2 from hemoglobin. p) The affinity of hemoglobin for O2 increases with increase of the CO2 concentration. q) The oxidation state of Fe in the functional myoglobin is +3. r) The acidic environment of an exercising muscle makes hemoglobin bind to O2 more strongly. s) A single amino acid substitution in the ß-chain of hemoglobin causes sickle-cell anemia. t) Myoglobin is an allosteric protein, whereas hemoglobin is not. u) The affinity of hemoglobin for O2 is regulated by an organic phosphate (2.3-BPG), whereas the affinity of myoglobin for O, is not. v) In the lung, the presence of higher concentration of O2 promotes the release of H' and CO,

Explanation / Answer

a)False,The most commonly seen form of DNA double helix in the cell is the B Form helix

b)True

c)False actually Daughter DNA is newly synthesized from the cleavage of parental dna into two parts each of these daughter DNA is the copy of parential DNA

d)False- Bases stalked in double stranded DNA absorb less UV light than single strand DNA

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