A spectrophotometer measures the transmittance or the absorbance, or both, of a

Question

A spectrophotometer measures the transmittance or the absorbance, or both, of a particular wavelength of light after it has passed through a liquid sample. Before the transmittance or absorbance of the sample is measured, a cuvette filled only with solvent, called the blank, is placed in the spectrophotometer. Select the following step that is NOT necessary to complete before a cuvette is placed into the spectrophotometer O O O O Inspect the cuvette for any scratches or cracks. If present, replace the cuvette. Write, in ink, either sample or blank on the side of the cuvette to keep track of them Make sure the cuvette is oriented in the spectrophotometer in the same way each time it is used. Make sure the cuvette is filled only 3/4 full to prevent spilling of the sample.

Explanation / Answer

Step B is not required. It is not required to write blank on the side of the cuvette. Infact you should not erite anything on the cuvette because it will affect the spectrum.

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