Spectroscopy 1. (1 point) Why in Raman spectroscopy are the spectra shown in wav

Question

Spectroscopy 1. (1 point) Why in Raman spectroscopy are the spectra shown in wavenumber instead of wavelength? (1 point) Calculate the photons emitted per second from an Alexa488 dye pumped at 100 W/cm 2 2. find the photon emission first 3. (1 point) use the Perrin equation to explain why the fluorescence anisotropy of fluorescein in solution is nearly zero, whereas for GFP it is not 4 (1 point) Why does the gain of a PMT change so rapidly (non - inearly) with the voltage between dynodes? Lab portion NOTE: Please see the attached excel data that I obtained during lab to answer the following 2 questions (3 points) Make an UV absorbance measurement of the sample provided and report the concentration. The sample content may vary and will be make know to you at the time of the experiment-Describe all details in ~ ½ page report. Include a plot of the spectrum. 1. Ans: See excel files 2. 13 points) Measure the excitation and emission spectra of the sample provided. Attempt to identify the fluorophore. Describe all details in ~ ½ page report. Include a plot of the spectra. Ans: See excel files

Explanation / Answer

1. Raman spectra are shown in wavenumber in inverse centimetre instead of wavelength. This makes reading the spectra convenient as the wavenumber scales linearly with energy.

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