Answer in a few sentences for #1-3. These questions are based off a HPLC Determi

Question

Answer in a few sentences for #1-3. These questions are based off a HPLC Determination of Caffeine in Power Drinks. Procedure is here: Questions (Provide answers on additional sheets or in the Laboratory Notebook): (1) (2) (3) Why is it crucial to filter your energy drink unknown sample prior to HPLC analysis? What experimental benefits arise from utilization of an autosampler? How would you be able to determine if there was a chemical contaminant in your energy drink that coeluted with your caffeine peak? What instrumental aspects could be changed to separate these coeluted chemicals?

Explanation / Answer

1) Ans:

If any solid particles or impurities present in the sample will get remove by filteration,otherwise it will plug the chromatographic column. Unknown sample was filtered through a 0.45 ?m syringe filter with a 5 mL syringe. Therefore it is crucial to filter your energy drink unknown sample prior to HPLC analysis.

2) Ans:

Experimental benefits arise from utilization of an autosampler is  precise, reliable, and accurate injection for nanoliter to milliliter sample volumes. It provides fastest sample injection (10 seconds), which dramatically reduces the total cycle time.

3) Ans:

In chromatography combined with spectrometry, the signals are of complicated structure consisting of individual spectral peaks of caffeine. By changing column or changing mobile-phase composition we can seperate these coeluted chemicals.

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.