when measuring the %T either in setting the spectrometer in measuring the BLANK

Question

when measuring the %T either in setting the spectrometer in measuring the BLANK or a SAMPLE, the sample compartment cover must be a ways be closed. " it is left open the 6T will probably be Select one: a. Too high because in addition to the light transmitted there wil be extra light leaking in b. Too low because of dust which will settle on the sample c. Too low because the light passing through the sample will tend to leak out d. Too low because the detector wll now be reading light of various wavelengths

Explanation / Answer

%T is the percentage of the light transmitted by the substance relative to the reference BLANK.

%T for reference BLANK is 100% as the compund being tested is not present in reference BLANK.

If we place a compund in the cauvette, a certain amount light is abosrbed by the compound and the %T is less than the 100%. Taking this as reference when we keep the compartment cover is kept open, light passing through the sample will tend to leak and %T will be too low

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