Humans are aerobic organisms. If so why do we worry about being infected by an a

Question

Humans are aerobic organisms. If so why do we worry about being infected by an anaerobic organism? A swab for culture is received from a doctor's office. The staff forgot to mention the source from where the swab was taken and the clinic was closed for next few days. What different selective/differential media may be helpful to you to identify the organism. Explain very briefly your rationale for using the particular culture media.? Agar and gelatin are used as a solidifying agent for bacteriological media. Why is gelatin less suitable than the agar? A catalase test is performed on an isolated colony on a blood agar plate and bubbles appear. The test is repeated on another isolated colony form the same plate. This time there are no bubbles. How can you explain the difference in the results? You are given a tube of contaminated water. The number of bacteria in the tube is 100 many to count. You prepare a tenfold dilutions in 5 tubes, so that tube #1 has 1: 10 dilution, tube #2 has 1: 100 dilution, tube #3 has 1: 1000 dilution, tube #4 hat 1: 10, 000 dilution and tube #5 has 1: 100, 000 dilution. The second tube has 210 cells and the third tube has 26 colonies. What is the number of bacteria per ml in the original sample? Several individuals have food borne poisoning due to Salmonella from a common source. Later several individuals went to the local emergency rooms of the hospital complaining of similar symptoms. How could you determine and be sure that these individuals have the same infection? Distinguish between alpha-and beta-hemolysis? How would you differentiate between Staph.aureus and Strep.pneumoniae? Why are females more prone to UTI than males? What would be the effect of a urine sample being left at room temperature overnight before transported to the laboratory for culture?

Explanation / Answer

Anaerobic organisms can cause infection in the human body actually through out the human body. There are two types of anaerobic organism, one is facultative anaerobe and obligate anaeobe. Obligate anaerobe can not grow in the presence of oxygen but they can infect where there is almost no oxygen like deep wounds, deep tissues and internal organs. These infections are characterized by abscess formation that is foul smelling pus and tissue destruction. This is possible because of necrosis or poor blood supply and this enhances the growth of anaerobic bacteria. Facultative anaerobes can grow even in presence of oxygen and they cause infections in human frequently. That is why even if we are aerobic organism, anaerobic organisms can infect humans.

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