/ibiscins/mod/ibis/view.php?id# 2656460 of AlabarX Sapling Learming Map Determin

Question

/ibiscins/mod/ibis/view.php?id# 2656460 of AlabarX Sapling Learming Map Determine the minimum number of theoretical plates, N, required to achieve baseline separation witha resolution that is desirable for quantitative analysis of two compounds with an adjusted relative retention, a, of 1.060 and retention factors of 9.30 and 9.86 plates in an attempt to increase the resolution of the separation, a researcher increased the column length. True or false, increasing the column length wil increase the resolution of the separation? O True O False

Explanation / Answer

1) Relationship between resolution®,number of theoretical plates (N) and retention factors is given by

R=1/4 *(N)^1/2 *(a-1/a)*(k/1+k)  where a=separation factor =1.060

k=retention factor of rear peak as both peak are peak width)=9.86

For complete separation R=1.5

1.5=1/4 * (N)^1/2 *(1.060-1/1.060)(9.86/1+9.86)=(N)^1/2 * 0.0129

(N)^1/2=1.5/0.0129=115.38

N=1.07=11

N=11

resolution is proportional to theoretical plate number (N) which in turn is proportional to column length so increasing column length increases N and hence efficiency

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