* I mainly need the fourth answer. Thank You* Look at the following amino-acid s
ID: 102066 • Letter: #
Question
* I mainly need the fourth answer. Thank You*
Look at the following amino-acid sequences from two warm-water species and one cold-water species and identify where changes in the sequence occurred. Line 1: C. punctipinnis:Temperate (cold-adapted)
Line 2: C. caudalis:Tropical(warm-adapted)
Line 3: C. xanthochira: Tropical (warm-adapted) 151
151
GFERHRVIGS GTNLDSARFR HIMGEKLHLH PSSCHGWIVG EHDGSSVPVW
GFERHRVIGS GTNLDSARFR HIMGEKLHLH PSSCHGWIVG EHDGSSVPVW
GFERHRVIGS GTNLDSARFR HIMGEKLHLH PSSCHGWIIG EHDGSSVPVW
201
SGVNVAGVSL QGLNPQMGTD GDSEKWKEVH KLVVDGAYEV IKLKGYTSWA
SGVNVAGVSL QALNPQMGAE GDSENWKAVH KQVVDGAYEV IKLKGYTSWA
SGVNVAGVSL QGLNPKMGAE GDSENWKEVH KLVVDGAYEV IKLKGYTSWA
251
IGMSVADLVE SIMKNLHKVH PVSTLVQGMH GVKDEVFLSV PCVLGNSGLT
IGMSVADLVE SIMKNLHKVH PVSTLVQGMH GVKDEVFLSV PCVLGNSGLT
IGMSVADLVE SIMKNLHKVH PVSTLVQGMH GVKDEVFLSV PCVLGNSGLT
301
DVIHMTLKPE EEKQLVKSAE TLWGVQKELT
DVIHMTLKPD EEKVLVKSAE TLWGVQKELT
DVIHMTLKPD EEKQLVKSAE TLWGVQKELT
Which amino acids changed between the three species? List four of them below, using the number of the amino acid. Example: Amino acid 189 changed from valine to isoleucine in a warm adapted species.
1) _______________________________________________________________
2) _______________________________________________________________
3) _______________________________________________________________
4) _______________________________________________________________
What were the changes in the chemical properties of the R-groups? Use the amino-acid chart to in the Powerpoint file to answer this question. Example: Both valine and isoleucine are nonpolar.
1) _______________________________________________________________
2) _______________________________________________________________
3) _______________________________________________________________
4) _______________________________________________________________
How might the changes that you identified affect the protein’s structure? Example: The change would likely have no effect. Neither valine and isoleucine would promote hydrogen bonding with water, because their R groups are nonpolar and thus hydrophobic.
1) _______________________________________________________________
2) _______________________________________________________________
3) _______________________________________________________________
4) _______________________________________________________________
4. Assuming that the mutations are adaptive, how should the changes that you identified affect the stability of the protein at low and high temperatures?
Explanation / Answer
Amino acids changed :
1. Amino acid 151 changed from valine to isoleucine in a warm adapted species.
2. Amino acid 201 changed from glycine to alanine in warm adapted species.
3. Amino acid 201 changed from glutamine to lysine in warm adapted species.
4. Amino acid 201 changed from glutamic acid to aspartic acid in cold adapted species.
Changes in chemical properties of R-groups :
1. Both valine and isoleucine are nonpolar.
2.Both glycine and alanine are nonpolar.
3.Glutamine is an uncharged polar group and Lysine is a charged polar group.
4. Both glutamic acid and aspartic acid are charged polar group.
Changes that affect the protein's structure :
1 and 2 : The change would have no effect.
3. polar groups are found on the surface of the protein, since both glutamine and lysine are polar, the change would have no such effect.
4.The change have no effect, since both glutamic acid and aspartic acids are negatively charged R groups.
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