1. What class of enzyme catalyzes both of the reactions of ethylene glycol? 2. T
ID: 1017983 • Letter: 1
Question
1. What class of enzyme catalyzes both of the reactions of ethylene glycol?
2. The following is a segment of the template strand of human BRCA1 gene : 3 TGG AAT TAT CTG CTC TTC GCG 5'. Write the corresponding mRNA segment
4. Match each of the following processes with one of the items
5. Endorphins are polypeptide that reduce pain. What is the amino acids order for the endorphins leucine enkephalin, which has the following mRNA? Drag the appropriate labels target. Note: not all labels will be used.
6. Why are E.coli bacteria used in recombinant DNA procedures?
7. Match the type of cellular control with the description.
8. State whether each of the following
components is present in DNA only or both DNA &RNA:
9. How is a gene for a protein inserted into a plasmid?
Explanation / Answer
1. Class 1. Oxidoreductases
2. 3' TGG AAT TAT CTG CTC TTC GCG 5' sequence on DNA is decoaded as 5' ACC UUA AUA GAC GAG AAG CGC 3' on m-RNA
4. recombinent DNA technology
5. Start Met-Tyr-Val-Gly-Phe-Leu Stop
6. in E.coli thereare around 4000 nuclotide sequences, and all these are explored, sequenced, and the availibility of extra chromosomal plasmides material makesit as a good genetic engineering tool for recombinent DNA technology purpose.
8. deoxycytidine monophosphate is found only in DNA.
Adinine and phosphate are found in both DNA and RNA.
Ribose is found in RNA only
9. This method of insertion of a gene for a protein intoa plasmid is a part of molecular cloning experiments. In standard molecular cloning experiments, the cloning of any DNA fragment essentially involves seven steps: (1) Choice of host organism and cloning vector, (2) Preparation of vector DNA, (3) Preparation of DNA to be cloned, (4) Creation of recombinant DNA, (5) Introduction of recombinant DNA into host organism, (6) Selection of organisms containing recombinant DNA, (7) Screening for clones with desired DNA inserts and biological properties.
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