How many \"cross-overs\" are required to determine the order of 3 genes? A. Sing
ID: 101433 • Letter: H
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How many "cross-overs" are required to determine the order of 3 genes? A. Single B. Double c. More than 10 E. None () In a single, how many remain parental (non-crossover)? A. 1 B. 2 C. 3 D. 4 Identify for "3-point mapping": A. parents for all 3 genes B. Phenotype class must reflect genotype of parents C. sufficient number of offspring must be produced D. All of the above What is the basis of 'mapping" genes? A. Crossing over B. Synopsis C. Linkage D. Centromere absence What is produced in "greater" number in F2 phenotypes? A. non-crossover B. Single crossover(SCO) C. Double crossover (DCO) D. All the above What is produced in "lowest" number in F2 phenotypes? A. non-crossover B. Single crossover(SCO) C. Double crossover (DCO) D. All the above Calculate "coefficient of coincidence (C) if number of "observed" DCO is 3 an "expected is 6: A. 0.5 B. 2 C. 3 D. 9 interference (I) in the equation above: 0.5 B. 2 C. 3 D. 9 A " complete" interference occurs when: A. No DCO B. Observed DCO fewer than expected C. Observed DCO more than expected D. All of the above "Cystic fibrosis" is a good example of: A. Short repetitive sequence (microsatellite) Which of the following transduction methods target ONLY certain genes to be A. Regular B. Generalized C. Specialized d. None of the above By what structure factor are transferred from one to anther bacterium during conjugation? A. Pseudopodium B. Tentacles C. D. Plus A mutant bacterium for a particular nutrient cannot grow in a medium that lacks that nutrient is known as: A. Autotroph B. Heterotroph C. Auxotroph D. All of the above The bacterial cells that can picked naked DNA from outside (extracellular) during transformation are known as? A. Recombinant cell B. Genetically engineered cells D. Helper cell If the plasmid DNA "F factor ()" incorporated into the bacterial DNA, it is known as: A. An hfr (High frequency of recombination) cell B. F^+ cell C. F cell D. F cellExplanation / Answer
Answer 3(b) Double crossover is required to determine order of 3 genes.
Answer 4(a) 1 chromatid remains non crossover, because in single crossover exchange occurs only at one locus.
Answer 5(d) all of the above are true for 3 point crosses
Answer 6(C) linkage is basis of mapping genes, one can map genes on the basis of linkage analysis
Answer 7(a)non crossovers or parental types are greatest in number.
Answer 8(c) double crossovers are lowest in numbers always whereas, parental types are greatest in numbers
Answer 9(a) 0.5; C.O.C.= observed DCO/expected DCO= 3/6=0.5
Answer 10(a) interference = 1- C.O.C= 1-0.5=0.5
Answer 11(a) No DCO, a compete interferece occurs when there is no double recombinants i.e., DCO=0;thus COC=0 hence, interference= 1-COC=1i.e., complete interference
question 12 to 15 are not legible due to page fold and bad picture quality.
Answer 16(c) F'(prime) conjugation gives merozygote.
Answer 17(c) specialized transduction, transduce only some of the genes not all which is the case in generalized transduction where whole set of genes are transduced.
Answer 18(c) pilus is a structure which is formed during conjugation it is also called as sex pili and made up of proteins called pilin, it is a tube like structure which helps in transfer of F factor among conjugates.
Answer 19(c) Auxotrophs. Auxotrophs are mutants which can not grow without required nutrient in minimal media
Answer 20(c) these type of cells are called as competent cells
Answer 21(a) an Hfr cell, when F+ cell conjugates with Fcell , the factor become integrated into its chromosome and a high frequency recombinant is formed called Hfr Cell.
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