2.) The severity of the disease (in our example from CFTR) is dependent on... a)
ID: 101206 • Letter: 2
Question
2.) The severity of the disease (in our example from CFTR) is dependent on... a) How close to the transcription start site the mutation occures b) The final quantity and functionality of the protein in the plasma membrane c) The location of the misfolded protein d) The stability of the mislocalized protein 3.) An example of protein trafficking is a) Anacscent protein being translated and translocating through a translocon b) A protein attached to a COPII vesicle being returned to the cis-golgi c) Dynamin pinching off a vesicle d) The golgi modifying a protein such that the M6P signal is "active" 4.) Since CFTR is an ion channel, after it is folded correctly into the membrane but before it is trafficked to the plasma membrane, where could it be sending Cl- ions? a) The cytosol b) The extra-cellular environment c) The golgi d) The nuclus 5.) Which protein detects a hydrophobic transmembrane region of a nascent protein? a)BiP b)ribosome c)SRP d)translocon 6.) How do SNAREs help vesicle membranes fuse? a)They are transmembrane proteins so they merge the membranes when they meet b)They complex to bring the membranes closer together c)They maintain specificity so only the correct membranes can fuse d)They target the vesicle to the optimal membrane fusion location 7.) Why is it important that signal sequence are on the N-terminal end of a protein? a)The C-terminal end would not fold properly with a signal sequence on it b)The cell can "see" what the protein is before sending it somewhere c)The protein will get to the right organelle faster if the signal sequence is on the N-terminal d)The signal sequence can determine where the protein is translated 8.) Lipids are synthesized a)In the cytosol b)In the ER c)In the Golgi d)Whereever they are required 9.) The exocytosis pathway is required for (check all that apply): a)Build the lipid bilayer b)Deciding which proteins are membrane bound c)Membrane stability d)Moving membrane bound proteins to the plasma membrane e)Protein translocation f)Secreting proteins 10.) Order the events of the UPR a)BiP binding the Ire1 b)Bip binding to unfolding proteins c)Hac1p translation d)Ire1 dimerization e)KAR2 translation Thank You
Explanation / Answer
2. b, The final quantity and functionality of the protein in the plasma membrane
CFTR is characterized by a wide variety of mutations such as class I, II , III, IV , V. These are categorized based upon the impact of the mutation on the fate of the CFTR protein. For e.g. class I mutation is defined as impairing the translation of the protein and is caused by the creation of premature stop codons which leads to the production of truncated proteins and hence no functional CFTR at the plasma membrane.
3. b, A protein attached to a COPII vesicle being returned to the cis-golgi
4.c, golgi
5. c, SRP
SRP binds to the signal sequence of a newly synthesized peptide as it emerges from the ribosome. This binding leads to the slowing of protein synthesis known as "elongation arrest," a conserved function of SRP that facilitates the coupling of the protein translation and the protein translocation processes.
6.b, they complex to bring the membranes closer together
v-SNARE and t-SNARE proteins present on membrane of vesicle and target respectively combines to form a trans-SNARE complex.
8. b, ER and c, golgi
SER is responsible for the majority of lipid synthesis. However ER and Golgi apparatus together constitute the endomembrane compartment in the cytoplasm of eukaryotic cells. And this endomembrane compartment is a major site of lipid synthesis.
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