The protein catalase catalyzes the reeaction described by: The protein catalase

Question

The protein catalase catalyzes the reeaction described by:

The protein catalase catalyzes the reaction described by 2 H_2 O_2(aq) rightarrow 2H_2 O(l) + 0_2(g) and has a Michaelis-Menten constant of K_m = 25 mM and a turnover number of 4.0 times 10^7 s^-1. The total enzyme concentration is 0.019/Art and the initial substrate concentration is 5.29 muM. Catalase has a single active site. Calculate the value of R_max (often written as V_mnx) for this enzyme. Calculate the initial rate, R (often written as V_o), of this reaction.

Explanation / Answer

For the given data

Rmax = turnover number x enzyme concentration = 4 x 10^7 s-1 x 1.9 x 10^-5 mM = 760 mM.s-1

R = Rmax([S]/([S]+Km))

    = 760 mM.s-1(5.29 x 10^-3 mM/(5.29 x 10^-3 mM + 25 mM))

    = 0.161 mM.s-1

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