Which of the activities of DNA Polymerase I is most important in removing the pr

Question

Which of the activities of DNA Polymerase I is most important in removing the primer? a. Polymerase activity. b. Ability to nick intact double stranded DNA c, .5' rightarrow 3' exonuclease. D. 3'rightarrow 5' exonuclease. e. None of these is used for primer removal. Which of these proteins is used less for leading strand replication than for lagging strand replication? a. DNA polymerase b. DNA-binding protein c. DNA ligase d. DNA gyrase The mechanism of breakage and reunion of DNA strands during recombination was proposed by: a. Messelson and Stahl b. Messelson and Weigle c. Robin Holliday d. Francis Crick

Explanation / Answer

Ans 18) None of these is used as for the removal of primer.

Ans 19) c) DNA ligase. This is because the synthesis of lagging strands form as Okazaki fragments. These fragments are joined by the DNA ligase to form a single continuous strand. But the synthsis of leading strand is continuous. So, DNA ligase is not so much required in leading strand.

Ans 20) B) Messelson and Weigle. They proposed that the breakage and reunion of DNA srands on recombination in lamda phage.

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