Which of A. Coomassie dye was used B. Coomassie dye was ned to stais and viualin

Question

Which of A. Coomassie dye was used B. Coomassie dye was ned to stais and viualine pro Call of the above statements are omt D none of the ahove statoments ae regarding the Coomassile dye in the comect 10. How do A. 20g B. 5g C 0.2 D. 10 E I have so know the molecular weight of SDS to answer such question F none of te above you prepwo a 50nd solution of 20% (w/v) Sedium Dykyisemer esosi? How mah SOS should you uw? Bonus Question You were running an SDS-PAGE The dye fromt (bromophenol blue dye) was half way in the gels when there was a power outage at UMES. The power outage lasted many hours and this experiment was very important for your thesis an thus, you unplugged your gel system (the gel running apparatus) and ran with your gel apparatus to another UMES building to use the emergency power outlet. You plugged the power electrodesiconnection terminals and started running your gel system. However, when you checked your gel again, you soticed that the dye front was moving in the opposite direction (oward the upper part of the gel). What is the most likely explanation? A. there was something wrong with the emergency power when you plugged the system in again, you mistakenly reversed the electric polarity. That is to say, you did not plug the electrodes into the correct positive and negative outlets C. gels should be run continuously and, thus, once interrupted by power outage, proteins would reverse direction D all of the above E. none of the upowe

Explanation / Answer

The answer to the question 9 is Option C - all of the above statements are correct.

How do you prepare a 50 ml solution of 20% (w/v) Sodium Dodecyl Sulfate (SDS)? How SDS should you use?

We have to prepare 50 ml of 20% (w/v) SDS.

First, we have to understand what w/v stands for. It stands for weight per volume.

In the given problem, we have to prepare 20% w/v. That means 20 grams of SDS in 100 ml of water gives 20% (w/v) SDS in 100 ml.

Please note that the problem says we have to prepare 50 ml of 20% (w/v) SDS. So, we have to weigh10 grams of SDS and dissolve in 50 ml of water.

The correct answer is option D. 10 g

The answer to the bonus question

In PAGE, SDS is used to add negative charge uniformly to the protein which we are studying. While performing the SDS-PAGE experiment, wells containing proteins must be placed in the cathode (negative polarity). When power is supplied, protein moves from the cathode to the anode.

From the above explanation, it is very clear that the dye front was moving in the opposite direction due to the reversal of electric polarity.

So, the correct answer is Option B - when you plugged the system again, you mistakenly reversed the electric polarity.

Related Questions

Navigate

• Browse (All)
• Browse W
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.