PLZ HELP :) What is likely to happen If you spot too much material on a TLC slid

Question

PLZ HELP :)

What is likely to happen If you spot too much material on a TLC slide? "Briefly Explain" What is likely to happen If you spot too little material on a TLC slide? "Briefly Explain" If you developed a TLC slide (plate) with ethylene glycol (i.e., HOCH_2CH_2OH) and the material (sample) did not move, what can you say about the polarity of the material (i.e., sample)? "Briefly Explain" With respect to question 3 above, what solvent or solvent type might you try in order to move the compound on the TLC slide? "Briefly Explain" How might a mixture of compounds be separated and Isolated in pure forms using TLC? "Briefly Explain" In one TLC preparation ethylene glycol (i.e., HOCH_2CH_2OH) is used as the eluting solvent and in the other, pentane (H_3C(CH_2)_3CH_3) is used. When ethylene glycol is used, the solvent front as well as the sample moves on the TLC plate very slowly, and when pentane is used, the solvent front moves very quickly on the TLC plate but the sample moves very slowly ? "Briefly Explain the interactions between the mobile phase and stationary phase, and between the stationary and sample, as well as the interactions between the mobile and sample." You can ask your instructor advise.

Explanation / Answer

1. Spotting too much material should be avoided, because this will deteriorate the quality of the separation considerably (‘tailing’). The spots should be far enough away from the edges and from each other as well.

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