Organic chemistry II Lab.--Separation and Identification of Methyl of Fatty Acid
ID: 1007652 • Letter: O
Question
Organic chemistry II Lab.--Separation and Identification of Methyl of Fatty Acids by Gas Chromatography.Please, answer the following Questions that are from the above Lab.: 1) a) What is the purpose of the BF3 used in the esters fixation step. b) Why is helium gas used as a carrier gas c) How would the chromatographs differ for an injection of 1 uL as compare to 2 uL? d) How would the chromatographs differ from a smooth injection to an injection that was not done in a smooth motion? e) what would happened to the relation time of a compound if the following changes were made? 1. decrease the flow of the carrier gas 2)increase the Tem. of the colum. 3) increase the length of the column. Organic chemistry II Lab.--Separation and Identification of Methyl of Fatty Acids by Gas Chromatography.
Please, answer the following Questions that are from the above Lab.: 1) a) What is the purpose of the BF3 used in the esters fixation step. b) Why is helium gas used as a carrier gas c) How would the chromatographs differ for an injection of 1 uL as compare to 2 uL? d) How would the chromatographs differ from a smooth injection to an injection that was not done in a smooth motion? e) what would happened to the relation time of a compound if the following changes were made? 1. decrease the flow of the carrier gas 2)increase the Tem. of the colum. 3) increase the length of the column.
Please, answer the following Questions that are from the above Lab.: 1) a) What is the purpose of the BF3 used in the esters fixation step. b) Why is helium gas used as a carrier gas c) How would the chromatographs differ for an injection of 1 uL as compare to 2 uL? d) How would the chromatographs differ from a smooth injection to an injection that was not done in a smooth motion? e) what would happened to the relation time of a compound if the following changes were made? 1. decrease the flow of the carrier gas 2)increase the Tem. of the colum. 3) increase the length of the column.
Explanation / Answer
a)
b) In gas chromotography, the mobile phase is carrier gas. Helium gas is used because it is inert and unreactive.
c) I think there wont be any difference if you inject 1 uL or 2 uL.
d) The sample needs to be injected smoothly (uniform injection) If this does not happen it may give some error in retention time
e) What happens to the retention time
1. If decrease the flow of carrier gas there will be increase in retention time. with a good separation because as the flow of carrier gas is reduced the sample will interact with stationary phase.
2. Increase the termperature of the column will result in low retention time. This is because as the temperature increases the poor separation will takes place. It needs to be interacted with stationary phase.
3. Increase the length of the column the retention time increases because the gas will travel long distance. The longer the column improves the separation.
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