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1. Why is it important that a pencil rather than a ball-point pen or marker be u

ID: 1001358 • Letter: 1

Question

1. Why is it important that a pencil rather than a ball-point pen or marker be used to mark the TLC plate prior to development? 2. A TLC plate is developed in a solvent system containing 10% Ethyl Acetate -Hexane. After visualization, it is found that the spot did not move from the origin. What might you do to get the spot to move up the plate? 3. The three compounds below were spotted on a TLC plate and developed to give the TLC plate shown. Which compound corresponds to which spot? OH 4. Calculate the Rf value for spot B in the TLC plate shown above.

Explanation / Answer

1. Because carbon (graphite) is insoluble in eluent, whereas, ink could be dissolved into eluent.

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