Which of the reagents could be used to differentiate between solution A (whole m
ID: 99845 • Letter: W
Question
Which of the reagents could be used to differentiate between solution A (whole milk) and solution B (skim milk)? Explain your answer. A clear solution is mixed with Biuret's reagent and the resulting solution is blue. What do you know for sure about this solution in regards to what molecules are present/absent? If you were given a starch solution and a solution that consisted of a mixture of starch and albumin, could you use biuret's reagent to distinguish them? Why or why not? List the macromolecule(s) present in each of the four unknown solutions: Unknown A: Unknown B: Unknown C: Unknown D: How were you able to determine if the unknowns contained a mixture of macromolecules?Explanation / Answer
6) Gerber Method
The whole milk is mixed with sulphuric acid and iso-amyl alcohol in a special Gerber ube, allowing dissolution of the protein and release of fat. The tubes are centrifuged and the fat rising into the calibrated part of the tube is measuredas a percentage of the fat content of the milk sample. This test is not possible in skimmed milk as they are free from milk fat..!!
8) yes, we can distinguish between the starch solution and (starch + albumin) solution by using biuret reagents. Biuret reagents is used to identify peptide bonds present in the amino acid. Albumin is a amino acid it contains peptide bonds. Hence, when biuret is given to the mixture of (starch and Albumin) the biuret reacts with Albumin peptide bonds gives purple colour which is not found in the case of only starch solution..!!
7) the biuret reagents is itself blue in colour due to the presence of copper in it. When reacted with the solutions remains blue Hence no chemical reaction took place. Hence it signifies the absence of protein (peptide bonds) in the solutions..!!
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.