Use the terms below to match to their specific identities or roles in the Tet-of

Question

Use the terms below to match to their specific identities or roles in the Tet-off system:

Use the terms below to match to their specific identities or roles in the Tet-off system: Regulatory protein, tTA Tet-off regulator plasmid Tetracycline response element. TRE RAD52-eGFP A. The gene of interest (GOI) which we have constructed to be under the control of the TRE B. Binds to TRE and allows expression of GOI C. Carried stably in yeast and encoding the Tet-off regulatory protein D. In the presence of the transformed response plasmid and doxycycline this unbound site causes GOl to not be expressed.

Explanation / Answer

Regulatory protein,tTA - B. Binds to TRE and allows expression of GOI.

Tet-off regulator plasmid- C. Carried stably in yeast and encoding the Tet-off regulatory protein

Tetracycline resonse element, TRE- D. In the presence of the transformed response plasmid and doxycyline this unbound site causes GOI to not be expressed

RAD52-eGFP- A. The gene of interest (GOI) which we have constructed to be under the control of the TRE.

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