E.coli cells were infected at high MOI with two strains of T4 virus. One strain
ID: 99407 • Letter: E
Question
E.coli cells were infected at high MOI with two strains of T4 virus. One strain produced small (s) plaques, showed fast lysis (f), and created cloudy (c) plaques: the other strain was wild type for all three phenotypes. Following lysis, virus particles were recovered and used to infect E. coli cells at low MOI. Each plaque was assessed for size, the speed of lysis, and clarity. The resulting 10,000 plaques were distributed among eight genotypes, as follows: s f c 3340 s + + 530 + + + 3550 + f c 470 sf + 890 + f + 140 s + c160 + + c 920 Ignoring double crossovers, what is the estimated genetic distance (cM) between the genes causing small plaques and fast lysis? (Enter your answer as a whole number with no decimals (e.g. 8 or 5). Your answer will be assumed to be in cM).Explanation / Answer
The percentage of recembinants between s and f = 530+470= 1000/ 10000= 10 percentage
plus double recombinats= 160+140=300/10000= 3 percentage
= 10 +3= 13 cM
Answer= 13 cM
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