Ecoli Strain I and II were mated and these are the results. Observed results sho

Question

Ecoli Strain I and II were mated and these are the results. Observed results showed growth ulTelal Conjugation Student Guide 4. Tape your plates together. 5. Incubate the plates overnight at 37°C or for 24-48 hours at room temperature Record the expected growth results for the recombi space below the table. nant cells in Table 3. Explain your reasoning in the Recombinant Cell Growth on Antibiotic Plates Expected, if donor is strain: Il (plasmid transferred) Type of Plate Observed Results Il (chromosome transferred) LB+str LB+amp LB+str+amp LB+nal Table 3. Expected and observed growth response of recombinant cells to the antibiotics streptomycin, ampicillin, nalidixic acid, or streptomycin plus ampicillin. The "expected" results will vary depending on whether Strain I is the donor or whether Strain Il is the donor and transfers plasmid genes only, or chromosomal genes only.

Explanation / Answer

The results are showing that the strain I is the donor and strain II is the acceptor. The observed results have growth in every medium. Strain I only can survive when it gets amp resistance gene from plasmid and nalidxic acid resistant from chromosome. That is not possible in Hfr strains. However, if strain I transfers its gene to strain II, then the recombinants will get all resistivity. Therefore, the genes transferred from strain I to II. There was no error in results.

Related Questions

Navigate

• Browse (All)
• Browse E
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.