NEED TO SOLVE B on the blanks diluted concentration for each solution. Calculate
ID: 990433 • Letter: N
Question
NEED TO SOLVE B on the blanks
diluted concentration for each solution. Calculate the Volume of Cobalt (11) stock Volume of water (ml.) solution (ml.) Cuvette 0 Test tube 1 Test tube 2 Test tube 3 Test tube 4 Cuvette 5 0.00 2.00 4.00 6.00 8.00 Fill the tuvette-¾ full 8.00 4.00 2.00 0.00 | Fill the cuvette-34 full | B. Absorption spectrum. Before you perform the concentration study, it is first necessary to determine spectrum will be determined using only cuvette 5 and cuvette 0. The spectrum allows you to determine the best wavelength for the concentration study Familiarize yourself with the appropriate operation of the spectrophotometer With only deionized water (cuvette 0) in the compartment set the initial wavelength and calibrate the instrument so that 96T is 100% and Absorbance is Insert cuvette 5 and read the transmittance or absorbance. If the spectrophotometer only reads transmittance, calculate the Absorbance of the solution from the transmittance. Change the wavelength to the next value and calibrate with the deionized water insert cuvette 5 and read the transmittance. Repeat this procedure at all other wavelengths Plot the absorbance vs. wavelength and determine the analytical wavelength with the maximum absorbance, Amas. Phor the absotbance w. wavelength and determine the andyical wavelengthi B Absorption Spectrum Data Table 1: Absorption of cuvette 5 WayclengthTabsorbance Innu- ‰r absorbance wavelength nm) 383 400 460 470 502 525 590 635 660 700Explanation / Answer
The data you demanded to calculate is interdependent on each other. One of them should be known by the experiment and use of spectrophotometer at a set wavelength. If absorbance is known , then you can calculate the % transmittance as :
A = 2 - log10 %T
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