You want to artificially produce an enzyme, protein A, through genetic-engineeri

Question

You want to artificially produce an enzyme, protein A, through genetic-engineering. Although you can get your "engineered" bacteria to produce large quantities of A, the product is insoluble and inactive. Which of the following procedures might be worth trying to obtain a soluble and active enzyme. (multiple correct answers are possible) a) Make the bacteria produce A at a lower rate and in smaller amounts b) Dissolve the protein aggregate in urea, dilute and remove the urea c) Treat the insoluble aggregate with a protease. d) Make the bacteria produce chaperone proteins in addition to A.

Explanation / Answer

1)d)Make the bacteria produce chaperone proteins in adition to A .

Chaperones act as catalysts that facilitate assembly while not being a part of the assembled advanced. chaperones catalyse folding|biological process} by aiding the self-assembly process. they seem to operate by binding to and helpful unfolded or partly doubled polypeptides that ar intermediates on the pathway resulting in the ultimate properly doubled state.

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