Calibration curve and whether or not to use a concentration of 0 in curve? This
ID: 985971 • Letter: C
Question
Calibration curve and whether or not to use a concentration of 0 in curve? This concentration does not fit in with the other data points at all when graphed so I am assuming the answer is no. I just need good reasoning besides "looks good."
Here is the actual question:
Data Analysis 1.The following are data from a series of standard:s Morphine standards (in ng/mL) 25 0.377 0 10 20 0.686 0.619 450 50 0.530 0.467 1.321 0.036 0.036 0.035 0.035 0.038 0.037 avelen (nm) Prepare a standard curve for the standards using your answer to Post-Lab Question 2 Should you include the 0 ng/mL sample? Why or why not? (Provide a scientific or mathematical justification, not "because it looks better with/without it.")Explanation / Answer
If the shape of the calibration curve does not match the shape of the curve fit, its depends upon the reading you got, then it is very likely to give a non-zero intercept every time. So there is no significant for taking zero concentration for curve
If the concentration is zero does not give significant reading in the instrument. So the curve depends upon the different concentration with their corresponding readings will plot a curve. So the curve does not go through origin.
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