3.The pattern of inhibition for a steady-state ordered mechanism is shown below,

Question

3.The pattern of inhibition for a steady-state ordered mechanism is shown below, but if the reaction assumes equilibrium conditions versus steady-state conditions, the plot obtained by varying B with non-saturating A, adding only product Q to the assay tube, yields a C pattern than a NC plot.
(a)Explain the difference between “steady-state” and equilibrium conditions
(b) Why does this change yields particular patterns in both cases (explain for S-S and for equilibrium conditions) when B is varied with added Q . A + B<-----> P + Q

Explanation / Answer

  In an equilibrium a single process or reaction is occurring at the same rate in the forward and backward directions, so that over time, there is no net change in the composition of the system.

In a steady state, there is likewise no net change over time in the concentrations of particular components, but this is because they are being produced and degraded at constant rates.

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