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2. (10 pts) Circle True or False for each of the following statements about spec

ID: 982056 • Letter: 2

Question

2. (10 pts)  Circle True or False for each of the following statements about spectrophotometry: a. True / False:  Percent transmission is proportional to concentration of a chromophore. b. True / False:  The molar absorptivity of a chromophore is a constant for that chromophore, so if you know its value from one experiment you could use it in another experiment, given the same conditions. c. True / False:  The molar absorptivity of a chromophore is characteristic of that chromophore, and thus has the same value regardless of wavelength being monitored.   d. True / False:  Beer’s Law predicts that for a fixed path length, absorbance will be proportional to concentration for any value of absorbance or concentration. e. True / False:  A student needs to take an absorbance spectrum of a sample across all wavelengths from 250 to 700 nm.  You should advise the student that it will be faster to use a singlebeam spectrophotometer instead of a photodiode array spectrophotometer.

Explanation / Answer

a) Percent transmission is proportional to concentration of a chromophore- TRUE, the given statement can be derived from the Lambert's Law

b) The molar absorptivity of a chromophore is a constant for that chromophore, so if you know its value from one experiment you could use it in another experiment, given the same conditions. - TRUE, this value is the constant which we can use for other equations

c) The molar absorptivity of a chromophore is characteristic of that chromophore, and thus has the same value regardless of wavelength being monitored. - FALSE

It depends on the wavelength of the light used

d) Beer’s Law predicts that for a fixed path length, absorbance will be proportional to concentration for any value of absorbance or concentration - TRUE

e) A student needs to take an absorbance spectrum of a sample across all wavelengths from 250 to 700nm - TRUE

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