0000C hile in graduate school you develop an ELISA to test for the presence of h

Question

0000C hile in graduate school you develop an ELISA to test for the presence of harmful algae in ocean water. You tran several high school students to run this test on 1-ml samples ot around the world. Untortunatery, your students get confused and add 50 microiners of blocking solution to each sample of ocean water and both controls Aterward they perform the ELISA exactly shown in the video, tcluding the normal blocking solution step How w this afect your results? O The blocking solution will likely have no efflect on the ELISA resuts g. Fase POSITIVE results are ikely Samples that are negative may appear positive

Explanation / Answer

ans should be B) because as given data in question sample increases from 1 ml to 50 ml (as shown in the image but image in not very clear)so if antigen increases then amount of substrate hydrolysed will also be increase so amount of antigen is directly proportional to amount of substrate hydrolysed.

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