You run an ELISA for molecule X. You test your sample, a negative control, and a

Question

You run an ELISA for molecule X. You test your sample, a negative control, and a positive control. How would you interpret the following data? Positive control: The ELISA turns a very pale yellow. Negative control: The ELISA turns a very pale yellow. Sample: The ELISA turns dark yellow. The sample is negative for molecule X. The sample is contaminated. You cannot accurately interpret these data, and you need to rerun the ELISA. The sample is positive for molecule X. You run an ELISA for molecule X. You test your sample, a negative control, and a positive control. How would you interpret the following data? Positive control: The ELISA turns a very dark yellow Negative control: The ELISA turns a very pale yellow Sample: The ELISA turns dark yellow The sample is contaminated. The sample is positive for molecule X. The sample is negative for molecule X. You cannot accurately interpret these data, and you need to rerun the ELISA.

Explanation / Answer

Answer:

Part E: (c) You cannot accurately interpret these data, and you need to rerun the ELISA.

Explanation:

Since both the positive and the negative controls give the same color, the ELISA result for molecule X is not very reliable. Thus, the ELISA needs to be rerun.

Part F: (b) The sample is positive for molecule X.

Explanation:

The positive control gives a very dark yellow color which suggests the presence of the molecule at higher concentration. The negative control gives a very pale yellow due to the absence of the molecule. Since the ELISA results for molecule X is dark yellow, it denotes the presence of the molecule but at a lower concentration than in the positive control. So, the ELISA result for the molecule X is positive.

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