The complete genome sequence of cattle is available and can be used for mass spe

Question

The complete genome sequence of cattle is available and can be used for mass spectrometry-based proteomics analysis. You decide to use a tandem mass spectrometry approach to examine gene expression in udder tissue during lactation. (1). Describe an experimental approach for identifying proteins that are expressed during lactation, how the resulting data are interpreted, and how you related the genomic data and MS data. (2). You next want to compare protein expression in udder tissues during the stages of lactation and pregnancy. Describe an experimental approach for protein identification and quantitation and a strategy for comparing the resulting protein expression profile for pregnancy versus that for lactation.

Explanation / Answer

1.The tandem mass spectrometry in space should be used here as it is the separate elements that are physically separated and distinct. The resulting data will be compared with the results of the:

Genomic data and

MS data

To confirm the expression and quantification of increased levels of the proteins those are expressed during lactation.

2. An experimental approach for protein identification and quantitation of protein expression in udder tissues during the stages of lactation and pregnancy should be the use of tandem mass spectrometry in time.

An experimental approach for protein identification and quantitation should be the simple mass spectrometry experiments. Further, an experimental strategy for comparing the resulting protein expression profile for pregnancy versus that for lactation will be the use of tandem mass spectrometry in time.

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