A mixture of aspartic acid, methionine and arginine may be separated by electrop

Question

A mixture of aspartic acid, methionine and arginine may be separated by electrophoresis. What would be the ideal pH for conducting this separation, and how would the separation take place? The isoelectric points for Asp, Met, and Arg are 2.77,5.74, and 10.76, respectively. SmallCircle The optimum pH would be around 3.50. This is the best pH to conduct all electrophoresis. Separation at this pH means that Arg moves quickly toward the negative electrode, Met moves slowly to the negative electrode and Asp moves incredibly slowly toward the negative electrode, allowing for separation. SmallCircle The optimum pH would be around 1.00. At this pH all species would now be positively charged O and the separation would be determined by the size of the charged species traveling along the plate. SmallCircle The optimum pH would be around 5.74. the isoelectric point of Met. At this pH Met is neutral and O does not move Asp is negatively charged and moves toward the positive electrode and Arg is positively charged and moves toward tne negative electrode. SmallCircle The optimum pH would be around 5.74, the isoelectric point of Met. At this pH Met is neutral and (j does not move. Asp is positively charged and moves toward the negative electrode and Arg is I negatively charged and moves toward the positive electrode.

Explanation / Answer

An ideal pH for the separation of the three amino acids would be,

The optimum pH would be around 5.74, the isoelectric point of Met. At this pH Met is neutral and does not move. Asp is -vely charged and moves towards the postive electrode and Arg is +vely charged and moves towards the negative electrode

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