1. What is the mechanism of action for novobiocin? Select one: a. Interferes wit
ID: 97397 • Letter: 1
Question
1.
What is the mechanism of action for novobiocin?
Select one:
a. Interferes with S. niveus.
b. Interferes with ATPase activity
c. Inhibits cell wall synthesis
d. Inhibits DNA replication
e. Inhibits hemolysis
2.
Gamma-hemolysis on a blood agar plate would likely appear:
Select one:
a. As no change in the medium
b. Blue
c. Yellow
d. Green
e. As a clearing
3.
Identification of Gram-positive cocci: You perform a gram stain and notice that each spherical cell is isolated from other cells (i.e. not in clusters, chains, etc.). Which genus would you NOT expect your unknown bacteria to be (rarely found as single cells)?
Select one:
a. Streptococcus
b. Micrococcus
c. Enterococcus
d. Staphylococcus
4.
Which one of the following is NOT true about hemolysin?
Select one:
a. They often cause fevers
b. They are common in Streptococci and Enterococci bacteria
c. They are exotoxins
d. They can cause anemia
5.
The main difference between between type O and type S streptolysins is that type O:
Select one:
a. Expresses optimally under aerobic conditions
b. Expresses optimally under anaerobic conditions
c. Is not affected by oxygen
d. Can be destroyed by oxygen
6.
Identification of Gram-positive cocci: You have isolated a catalase-negative bacterium that doesn't grow well in 6.5% NaCl medium. Which test would you expect to give you a presumptive identification? (i.e. The first tests tell you the genus, which test would identify a species of that genus)
Select one:
a. PYR Test
b. Novobiocin Sensitivity Test
c. Coagulase Tube Test
d. CAMP Test
7,
Why was it important to grow both types of bacteria on the same plate for this susceptibility experiment?
Select one:
a. The bacitracin disks talk nicely to each other
b. To confuse students
c. To avoid wasting resources
d. To prevent for fluccuations in grown from media differences
Explanation / Answer
1) d. Inhibits DNA replication.
It acts by hindering energy transfer during the action of DNA gyrase which is involved in removing supercoils in the DNA during replication. Novobiocin interferes with the energy transduction by targeting the GyB subunit of DNA gyrase which catalysis the ATPase reaction.
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