1. What is the minimum zone of inhibition for the bacteria to be considered susc

Question

1.

What is the minimum zone of inhibition for the bacteria to be considered susceptible to novobiocin?

Select one:

c. 16 mm

2.

Identification of Gram-positive cocci: You execute an Optochin Test using a disc. You observe a clearing of 13 mm with surrounding confluent growth. Which of the following would you conclude?

Select one:

a. The test was double positive

b. The test was negative

c. The growth was insufficient

d. The test was positive

3.

Identification of Gram-positive cocci: Which genus of the following would likely test positive for catalase and grow in 6.5% salt?

Select one:

a. Enterococcus

b. More than one is correct

c. Micrococcus

d. Streptococcus

e. Staphylococcus

4.

Bacitracin is used for:

Select one:

a. Identifying unknown bacteria

b. Boo-boos

c. Preventing infection after skin cuts

d. Uh-ohs

5.

Identification of Gram-positive cocci: Which genus of the following would NOT likely grow in a candle jar?

Select one:

a. Micrococcus

b. Staphylococcus

c. More than one is correct

d. Streptococcus

e. Enterococcus

6.

Non-fastidious microbes would:

Select one:

a. Grow on blood agar only

b. Grow on TSA and blood agar plate, but not in any other medium

c. Grow on a TSA plate only

d. Grow on most types of agar plates containing nutrients

7.

The bacitracin peptide is produced:

Select one:

a. By chemical synthesis in a lab

b. Naturally by Bacillus licheniformis

c. Naturally by Micrococcus luteus

d. Naturally by Staphylococcus aureus

Explanation / Answer

1.ans: option C

A zone of inhibition surrounding the NB5 disc less than 16mm is considered resistant and a zone greater than or equal to 16mm is considered susceptible. Staphylococcus saprophyticus is resistant while other Staphylyococcus species are susceptible.

2. ans: option C

Growth was insufficent

A smaller zone of inhibition (< 14 mm) or no zone of inhibition indicates that the bile solubility test is required. It is important to remember that pneumococci are sometimes optochin-resistant.Any zone of inhibition less than 14 mm is questionable for pneumococci; the strain is identified as a pneumococcus only if it is bile soluble

3.ans: option A

A positive test is the presence of obvious bacterial growth in the medium, with or without a color change in the indicator. If the organism is bile-esculin-positive and grows in the 6.5% NaCl broth, the organisms are an ENTEROCOCCUS species. If the organism is bile-esculin-positive and fails to grow in the 6.5% NaCl broth, the organism is a group D streptococcus

4.ans: option C

Preventing infection after skin cuts.This medication is used to prevent minor skin infections caused by small cuts, scrapes, or burns. Bacitracin works by stopping the growth of certain bacteria. It belongs to a class of drugs known as antibiotics. This antibiotic only prevents bacterial infections

6. ans: option D

Grow on most types of agar plates containing nutrients.A fastidious organism is any organism that has a complex nutritional requirement. In other words, a fastidious organism will only grow when specific nutrients are included in its diet

7. ans: option B

Naturally by Bacillus licheniformis

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