Your instructor will show you how to use the gel documentation system to visuali
ID: 97393 • Letter: Y
Question
Your instructor will show you how to use the gel documentation system to visualize your gel and capture an image of the gel. Can you explain what has happened in each reaction (A-F) based on what you see on the gel? Let's take a look at each reaction. Drawing the DNA molecules will help you visualize the molecules being cut. Recall: Lambda DNA is a linear piece of DNA and is 48, 502 base pairs (bp) in length, i.e. 48.5kb. Plasmid pBR322 is a circular piece of DNA and is 4207 base pairs (bp) in length, i.e. 4.2kb. 1. Tube A (lane 3) has uncut linear DNA (lambda DNA) and tube D (lane 6) has uncut plasmid DNA (pBR322). What accounts for the difference in mobility of the DNA through the gel? 2. Tube B (lane 4) shows results of lambda DNA cut with Hind III. Lambda DNA is a linear piece of DNA. Based on your results, how many sites does Hind III cut lambda DNA?Explanation / Answer
1. The difference in mobility of the DNA through a gel is based on the size of the DNA. Bigger the size; slower is the mobility.
2. The number of sites Hind III cuts the lambda DNA is the number of bands that are seen in the lane four. Count the number of bands to get the number of sites.
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