Zucchini plants infected with zucchini mosaic virus (ZMV) produce small, mottled

Question

Zucchini plants infected with zucchini mosaic virus (ZMV) produce small, mottled fruit that cannot be marketed. The virus is carried from one plant to another by aphids. Traditional farmers will use insecticides to kill the aphids and reduce the viral infection. a) There is a gene that will confer resistance to the ZMV infection. Why would making GM zucchini with this gene be an advantage? b) Below is the template strand ZMV resistant gene, with the start codon indicated as the colored region. Add the CaMV 35S promoter and NOS terminator at the correct positions on this game. ZMV resistance gene _______ c) The lab that produced this GM zucchini claims that farmers will need to use less insecticide. Why is that claim valid?

Explanation / Answer

1. a) The making of GM zucchini with this gene is advantageous as it describes the identification of single reaction, polymerase chain reaction-based markers linked to the ZMV-FL resistance gene. In this study, we identified a marker ZMV-resistant polymorphism(ZRP) linked to the ZMV-FL resistance gene locus. The identification of a single nucleotide polymorphism within the ZRP marker for the parental genotypes allowed for the development of a sequence-characterized amplification region marker linked to the ZMV-FL resistance gene

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