Below is a chromatogram obtained tor a mixture of benzene, toluene, and ethylben

Question

Below is a chromatogram obtained tor a mixture of benzene, toluene, and ethylbenzene. The column was packed with octadecylsilane-modified silica particles, and the column temperature was 30 degree C. The mobile phase was isocratic with water/ACN (50/50). The flow rate was f 1.0 mL/min. What does each of the three peaks represent? If the analyte contains also n-propylbenzene, where will its peak appear? How does the chromatogram change when the flow rate is changed to 0.8 mL/min? How does the chromatogram change when the mobile phase is water/ACN (40/60)? How does the chromatogram change when the column temperature is 50 degree C? in the gradient elution, how does the mobile-phase composition change with time?

Explanation / Answer

1) 1 is ethyl benzene, 2 methyl benzene 3 is benzene and if propyl benzene would have been there ..then it will be on left of 1.

hello this link answers your conceptual questions from 3 to 6

http://www.chem.ucla.edu/~bacher/General/30BL/gc/theory.html

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