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1)Based on the gene, mRNA and protein sequences that follow, what type of mutati

ID: 96887 • Letter: 1

Question

1)Based on the gene, mRNA and protein sequences that follow, what type of mutation-polypeptide effect has occurred?

Normal gene:    TAC GCC GGC CCG AAA TTT ACT
mRNA:             AUG CGG CCG GGC UUU AAA UGA
Normal protein:   Methionine-Arginine-Proline-Glycine-Phenylalanine-Lysine

Mutated gene:   TAC GCC GGC CCG ATA TTT ACT
mRNA:             AUG CGG CCG GGC UAU AAA UGA
Protein:   Methionine-Arginine-Proline-Glycine-Tyrosine-Lysine

a)base addition: change in one amino acid

b)base substitution: no change

c)base substitution: change in one amino acid

e)base deletion: change in one amino acid

2)Based on the gene, mRNA and protein sequences that follow, what type of mutation-polypeptide effect has occurred?

Normal gene:    TAC GCC GGC CCG AAA TTT ACT
mRNA:             AUG CGG CCG GGC UUU AAA UGA
Normal protein:   Methionine-Arginine-Proline-Glycine-Phenylalanine-Lysine

Mutated gene:   TAC GCC GGT CCG AAA TTT ACT
mRNA:             AUG CGG CCA GGC UUU AAA UGA
Protein:   Methionine-Arginine-Proline-Glycine-Phenylanine-Lysine

a)deletion: silent or no change

b)substitution: change in one amino acid

c)substitution: silent or no change

3)List the sequence of nucleotides in mRNA molecule that would be produced from the following DNA template strand during transcription.

DNA: TAC CCT GAA TGC AAA CAT GAA ATT

4) What causes induced mutations? What causes spontaneous mutations?

Explanation / Answer

1) In the amino acid sequence, phenyl aslanine in normal protein is replaced by tyrosine in the mutated protein. The mutation has caused a change in amino acid. So, this is called as base substitution mutation, which causes change in one amino acid (option c).