4/12/2016 05:30 PM A 12/13 4/12/2016 10:47 AM Gradebook Print Calculator Periodi

Question

4/12/2016 05:30 PM A 12/13 4/12/2016 10:47 AM Gradebook Print Calculator Periodic Table Question 19 of 25 Map Sapling The data below were collected for an enzyme-catalyzed reaction. This graph is provided for your use only. It will not be graded. (It is not 020 037 necessary to use a graph to answer.) Click on the graph to 0.50 0.83 activate the Tools 222 2.00 menu 0.6 400 Choose "Point," and 3.08 click a place on the graph to place Determine V and Km for this reaction. the point 0.4 To move a point, choose "Select," and Number then drag the point Use "Select" to m M. S scroll over a point to maN see its coordinates, or to see the equation of a line Use the option 0.0 -2 -1 0 2 3 5 "Line" to draw a line A Previous ® Give Up & View Solution Check Answer Next Exit Hint

Explanation / Answer

Limewr-burk plot can be obtained from V= Vmax[S]/ (KM+S)

1/V= (KM+S)/VmaxS

1/V= (KM/Vmax)1/S +1/Vmax

So a plot of 1/V Vs 1/S gives a straight line whose slope is KM/Vmax and intercept is 1/Vmax

The plot is shown below.

The equation is

1/V= 0.500*1/S+0.2

So 1/Vmax= 0.2, Vmax= 1/0.2= 5 mM/s

KM/Vmax= 0.5

KM= 0.5*Vmax= 0.5*5= 2.5mM

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