You have carried out a genetic screen in a species of fish, and have identified

Question

You have carried out a genetic screen in a species of fish, and have identified a new gene in this organism. You have cloned and sequenced the gene, but do not know anything about the biochemical function of the gene product. Which of the following is the most sensible next step in order to get clues about the function? a. Purifying the protein product and determining its structure by x-ray diffraction b. Screening for small molecules that perturb gene function c. Using the BLAST algorithm to scan sequence databases for similar sequences d. Using MS/MS to sequence the gene product without the need for purification e. Mutating the gene and performing reverse genetics

Explanation / Answer

After genetic screening, in order to study the function of specific gene, the most sensible step is mutating the gene and perfirming reverse genetics (e). The reverse genetics inclides finding homologous sequences in a gene, location of gene expression, etc. It concentrate in generating mutant organisms and charecterizing its phenotype. Study of mutants is the best way to understand a gene function, as mutation is the process which interrupt cellular function.

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