Why is there no weak or strong mobile phase in size-exclusion chromatography? O

Question

Why is there no weak or strong mobile phase in size-exclusion chromatography? O based on the extent to which the analytes penetrate the pores of O charged surface of the sfationary phase. The composition of the O immobilized onto the support. These interactions are unaffected by O to separation so that the strength of the mobile phase remains The mobile phase does not affect the retention since retention is the support. The retention of the analyte is based on interactions with the mobile phase does not affect these interactions. The retention of an analyte is based on interactions with molecules the composition of the mobile phase. The composition of the mobile phase is changed from separation constant for each separation.

Explanation / Answer

Size exclusion chromatography works on the priciples size or molecular weight of the analyte/molecules.

The molecules which are able to penetrate the pores of stationary phase are retained and other molecules bigger than the pore size of stationary phase are washed away by mobile phase irrespective of composition of mobile phase.

Hence here mobile phase does not have any interaction in retention of analyte/molecules.

Hence answer is A.

Related Questions

Navigate

• Browse (All)
• Browse W
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.