1. By what process is tetrads formed? Are these cells haploid or diploid? 2. Wha
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Question
1. By what process is tetrads formed? Are these cells haploid or diploid?
2. What is the purpose of zymolyase?
3. Once you get growth on the YPD plates, you will be performing replica plating (the same as you as previously done). The purpose of this is to detect mutations. Due to the diploid cells’ genotype (see previous labs if you don’t remember), those cells will be able to grow on a SD+his plate. Will any haploid cells be able to grow on an SD+his plate? If so, provide the genotypes for each haploid that can. If not, why not? (hint – refer to your prediction on spore formation)
Explanation / Answer
A tetrad is formed by a process called synapsis, which is included in the whole process of meiosis. It occurs in the first stage of meiosis called prophase 1. Here two homologous chromosomes align together, each of which is made up of two sister chromatids. The binding of each together is with the help of synaptonemal complex. This complex facilitates the crossingover of nonsister chromatids. The tetrads are haploid, as they are the results of meiosis. The zymolyase is an impotant enzyme in the enzyme extraction and purification process. It has lytic capacity of cellwalls of yeast. It is used in the digestion of yeast cellwalls for the enzyme purification purpose. This enzyme is produced by the bacteria Arthrobacter luteus.
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