Refer to Figure 21-49 and Box 21-1 in Lehninger. What are the main substrate and

Question

Refer to Figure 21-49 and Box 21-1 in Lehninger.

What are the main substrate and the cosubstrate for the conversion of cholesterol to the intermediate, 20,22-Dihydroxycholesterol? Note: Answers are listed as: Main Substrate; Cosubstrate

Additional background information: Cholesterol is converted to pregnenolone by an enzyme complex comprised of a mixed-function oxidase and a desmolase. Desmolases are enzymes that catalyze the formation or breakage of carbon-carbon bonds.

Refer to Figure 21-49 and Box 21-1 in Lehninger. What are the main substrate and the cosubstrate for the conversion of cholesterol to the intermediate, 20,22-Dihydroxycholesterol? Note: Answers are listed as: Main Substrate; Cosubstrate O Cholesterol; O2 20,22-Dihydroxycholesterol: Adrenodoxin O Cholesterol; Heme O Cholesterol: NADPH Additional background information: Cholesterol is converted to pregnenolone by an enzyme complex comprised of a mixed-function oxidase and a desmolase. Desmolases are enzymes that catalyze the formation or breakage of carbon-carbon bonds. What is the role of cyt P-450 in the mixed-function oxidase? O To carry electrons that are passed on to the main substrate. O To provide a scaffold for catalysis. O To supply oxygen atoms for the hydroxylation of cholesterol. O To cleave the side chain of cholesterol.

Explanation / Answer

1) Cholesterol, NADPH

2) To carry electrons that are passed on to the main subsrate

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