1.To begin your study of chromatin structure in the organism W. washdit, you loo

Question

1.To begin your study of chromatin structure in the organism W. washdit, you look at the
DNase sensitivity of three actin genes you have identified. You have determined by RT-PCR that
ACT1 produces 90% of actin mRNA, ACT2 contributes 10%, and ACT3 is not expressed. You
probe the structure of the actin genes with a DNase I mapping experiment. You grow a culture of
W. washdit cells, isolate their chromatin, and digest it with various concentrations of DNase I.
After removing the proteins, you cleave the DNA with the restriction enzyme XhoI, run the DNA
on a gel and perform a Southern blot. You probe your blot with a radiolabeled probe that will
hybridize to all three actin genes, and your results are shown below. From this experiment, what
can you conclude about the correlation between chromatin structure and gene activity in W.
washdit?

Explanation / Answer

just do the revirse

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