In the following example regarding gene order via co-transformation frequnecy I

Question

In the following example regarding gene order via co-transformation frequnecy I amhaving a hard time understanding what the information actually means. I realize higher frequencies result in closer linkage and closer distance between alleles, however, I do not understand what the signifcance/relation is between the observation of two different versions. For example, it is my understanding that the percentages 3.00 and 0 are co-transformation frequencies between his and leu, how can one of them be 3 and the other be zero? is that not contradictory to linkage?

donor : his+ leu+ lac+ recepient: his-leu- lac-

Genotype of transformed cells percentage of all cells his+ leu+ lac+ 0.02 his+ leu+ lac- 0* his+ leu- lac+ 2.00 his+ leu- lac- 4.00 his- leu+ lac+ .10 his- leu- lac+ 3.00* his- leu + lac - 1.50

Explanation / Answer

The table describes about the number of co-transformants obtained when his+leu+la+ strain is used to transform his-leu-lac- strain. The table describes about all the possible genotype frequencies that are obtained. The percentage of cells represents the number of cells obtained in each genotype and not the linkage between genes.

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